Journal: Journal of Lipid Research

Article Title: Epigenetic control of microsomal prostaglandin E synthase-1 by HDAC-mediated recruitment of p300

doi: 10.1194/jlr.M072280

Figure Lengend Snippet: PTGES1 expression is reduced by HDACi (A, B) or depletion (C). A–C: qRT-PCR of the genes indicated (relative to β-actin) from murine carotid rings (A) and from human VSMCs (B, C). A: Effect of SAHA (2 μmol/l, 14 h). B: Effect of SAHA or apicidin (14 h), both relative to the control DMSO (Ctl). C: Effect of two different control scrambled RNAi (siSCRs) or siRNA against HDAC as indicated. *P < 0.05, n ≥ 3.

Article Snippet: Human VSMCs [human aortic smooth muscle cells #354-05a, human coronary artery smooth muscle cells #350-05a, and human carotid smooth muscle cells #3514-05a] were purchased from PELOBiotech (Planegg, Germany).

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: Nature Communications

Article Title: A CRISPR/Cas9-based enhancement of high-throughput single-cell transcriptomics

doi: 10.1038/s41467-025-59880-2

Figure Lengend Snippet: a Schematic figure of experimental workflow. A total of ~50,000 VSMCs were isolated from 4 donors and 2 tissue locations: coronary and pulmonary artery. b Normalized density of cells along the force directed layout (FLE) according to their artery of origin comparing 10x-V3 ( top ) with scCLEAN ( bottom ). c RNA velocity stream plots along pseudotime (CytoTRACE) mapped onto the FLE embedding. All detected genes are utilized to automatically calculate early pseudotime (black) to late pseudotime (yellow) according to the number of genes expressed. Black arrows indicate direction of transcriptional transition. d The total number of terminal states identified using CellRank comparing 10x-V3 ( left ) and scCLEAN ( right ). Number of lineages are shown. Optimal terminal states were found using Schur decomposition (gap in the real portion of eigenvalues) and then refined according to stationary distance of the coarse-grained Markov transition matrix (non-zero distance). e Only scCLEAN is shown (>1 lineage). Absorption probability of each cell belonging to each lineage and thus differentiating along pseudotime into either of the 2 terminal states, corresponding with lineage 1 ( top ) and lineage 2 ( bottom ). Yellow represents a 100% probability of that cell belonging to that lineage. f (left) Lineage absorption probability (lineage 1 = top, lineage 2 = bottom) plotted as a function of each cell’s position along the differentiation trajectory (ct pseudotime). Colors reflect tissue origin: coronary (red) or pulmonary (gray) artery. (right) Violin plots of absorption probabilities across coronary and pulmonary arteries for each lineage. g Heat matrix illustrating the relationship between the quantified absorption probability of each cell belonging to each lineage compared to the ground truth arterial identity of that cell. h Receiver operating characteristic (ROC) depicting the classification performance of identifying each artery to each lineage (both pulmonary and coronary lineage AUC = 0.99). Created in BioRender. Pandey, A. (2025) https://BioRender.com/r94y754 .

Article Snippet: Human coronary and pulmonary artery smooth muscle cells from four healthy donors were purchased from Cell Applications (San Diego, CA).

Techniques: Isolation

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Glutamate Exacerbates Traumatic Brain Injury‐Induced Acute Lung Injury Through NMDAR / ROS /Ca 2+ Signaling Pathway in Pulmonary Endothelial Cells

doi: 10.1002/kjm2.70087

Figure Lengend Snippet: Changes of serious lung injury after TBI‐ALI. (A) The TTC staining was performed to evaluate the brain injury volume. (B) The level of cytokines (TNF‐α, IL‐1β, and IL‐6) in peripheral blood. (C) The level of cytokines (TNF‐α, IL‐1β, and IL‐6) in BALF. (D, E) The wet/dry weight ratio and the protein concentration are detected. (F, G) Corresponding lung H&E staining and acute lung injury scores. (H) The expression of GluN1 in HPMVECs. Scale bar, 200 μm. Results represent the mean ± SEM of independent experiments of animals ( n = 8). * p < 0.05 versus Sham group; # p < 0.05 versus TBI group.

Article Snippet: Human pulmonary microvascular endothelial cells (HPMVECs) were purchased from Cell Applications Inc. (San Diego, CA, USA; Catalog No. 300K‐05a) and cultured in RPMI‐1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) with 1% penicillin–streptomycin and 10% fetal bovine serum (FBS, Gbico, Grand Island, NY, USA) at 37°C in a humidified atmosphere containing 5% CO 2 .

Techniques: Staining, Protein Concentration, Expressing

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Glutamate Exacerbates Traumatic Brain Injury‐Induced Acute Lung Injury Through NMDAR / ROS /Ca 2+ Signaling Pathway in Pulmonary Endothelial Cells

doi: 10.1002/kjm2.70087

Figure Lengend Snippet: Glutamate alters NMDAR/ROS/Ca 2+ pathway. (A) Cell viability (percentage of untreated control) of HPMVECs after the treatment of glutamate. (B) Immunofluorescence images showing ROS production in HPMVECs. (C) Comparison of Ca 2+ concentration in each group. (D) The levels of p‐NFAT and p‐p65 in cytoplasm and nucleus were tested by western blot. (E) Immunofluorescence stain of p‐NFAT and p‐p65 in nucleus. Results represent the mean ± SEM of independent experiments of cells ( n = 3). * p < 0.05 versus Sham group; # p < 0.05 versus Glu group.

Article Snippet: Human pulmonary microvascular endothelial cells (HPMVECs) were purchased from Cell Applications Inc. (San Diego, CA, USA; Catalog No. 300K‐05a) and cultured in RPMI‐1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) with 1% penicillin–streptomycin and 10% fetal bovine serum (FBS, Gbico, Grand Island, NY, USA) at 37°C in a humidified atmosphere containing 5% CO 2 .

Techniques: Control, Immunofluorescence, Comparison, Concentration Assay, Western Blot, Staining

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Retooling a Blood-based Biomarker: Phase I Assessment of the High-Affinity CA19-9 Antibody HuMab-5B1 for Immuno-PET Imaging of Pancreatic Cancer

doi: 10.1158/1078-0432.CCR-18-3667

Figure Lengend Snippet: A, There is a reduction in liver uptake of radioactivity with increasing antibody mass (error bars show one standard deviation). B, Maximum-intensity projection image demonstrates the effect in a patient injected with 3 mg antibody (left) and a (different) patient injected with 47 mg HuMab 5B1 antibody 2 h before injection of 3 mg radiolabeled HuMab 5B (right). C, Fused PET/CT, PET, and contrast-enhanced CT of PDAC liver metastases in a patient who received 47 mg of HuMab 5B1 before injection of the radiolabeled antibody. Small liver metastases measuring less than 5 mm (red arrows) are visualized with high contrast. SUVs values were 101.4, 70.7, and 53.4 g/ml, respectively. Arrowhead indicates splenic infarct.

Article Snippet: The fully human HuMab-5B1 antibody was manufactured for MabVax Therapeutics Holdings, Inc. (San Diego, CA) by Patheon Biologics (Groningen, Netherlands) under current good manufacturing practice (cGMP) guidelines and supplied in sterile vials to MSK.

Techniques: Radioactivity, Standard Deviation, Injection, Positron Emission Tomography-Computed Tomography